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hysphere c18 hd online spe  (Spark Holland)
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Bioanalysis methods for therapeutic oligonucleotides published during 2012–2022 (until August 2022).
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phenols  (Agilent technologies)
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Bioanalysis methods for therapeutic oligonucleotides published during 2012–2022 (until August 2022).
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Image Search Results


Bioanalysis methods for therapeutic oligonucleotides published during 2012–2022 (until August 2022).

Journal: International Journal of Molecular Sciences

Article Title: Bioanalysis of Oligonucleotide by LC–MS: Effects of Ion Pairing Regents and Recent Advances in Ion-Pairing-Free Analytical Strategies

doi: 10.3390/ijms232415474

Figure Lengend Snippet: Bioanalysis methods for therapeutic oligonucleotides published during 2012–2022 (until August 2022).

Article Snippet: 15 mer unmodified OGN [ ] , Human plasma , HySphere C18 HD online SPE (Spark Holland) Oasis WAX SPE (Waters) , A: H 2 O w/15 mM TEA/400 mM HFIP B: H 2 O/MeOH (50:50) w/15 mM TEA/400 mM HFIP(gradient elution) , 7 min (online SPE-LC–MS/MS); 6 min (UHPLC) , EC-C18 Poroshell 120 (3 × 100 mm, 2.7 μm, Agilent) (online SPE LC–MS/MS); Acquity OST C18 (2.1 × 50 mm, 1.7 μm, Waters) , Waters Acquity UPLC-Sciex API5000 triple quadrupole , 10 pM (online SPE-LC–MS/MS method) 0.05 nM (UHPLC–MS/MS method).

Techniques: Sample Prep, Allele-specific Oligonucleotide, Extraction, Targeted Proteomics, Clinical Proteomics, Modification